Bicyclic pyridine and pyrimidine p38 kinase inhibitors

ABSTRACT

The present invention discloses compounds corresponding to formula 
                         
wherein A, R, X, Y, R, R 1  and R 2  are as defined above, pharmaceutical formulations, methods of making and uses thereof.

CROSS REFERENCE TO RELATED INVENTIONS

This application claims the priority benefit under Title 35 U.S.C.119(e) of U.S. Provisional Applications Ser. No. 60/362,373, filed onMar. 7, 2002 and Ser. No. 60/430,508, filed Dec. 3, 2002, thedisclosures of which are herein incorporated by reference in theirentirety.

FIELD OF THE INVENTION

The present invention relates to certain bicyclic pyridine andpyrimidine derivatives as p38 protein kinase inhibitors. In particular,the present invention relates to 2-substituted amino-bicyclic pyridineand pyrimidine compounds, a process for their manufacture,pharmaceutical preparations comprising the same, and methods for usingthe same.

BACKGROUND OF THE INVENTION

Mitogen-activated protein kinases (MAP) is a family of proline-directedserine/threonine kinases that activate their substrates by dualphosphorylation. The kinases are activated by a variety of signalsincluding nutritional and osmotic stress, UV light, growth factors,endotoxin and inflammatory cytokines. One group of MAP kinases is thep38 kinase group which includes various isoforms (e.g., p38α, p39β, p38γand p38δ). The p38 kinases are responsible for phosphorylating andactivating transcription factors as well as other kinases, and arethemselves activated by physical and chemical stress, pro-inflammatorycytokines and bacterial lipopolysaccharide.

More importantly, the products of the p38 phosphorylation have beenshown to mediate the production of inflammatory cytokines, includingTNF, IL-1 and IL-6, and cyclooxygenase-2. Each of these cytokines hasbeen implicated in numerous disease states and conditions. For example,TNF-α is a cytokine produced primarily by activated monocytes andmacrophages. Its excessive or unregulated production has been implicatedas playing a causative role in the pathogenesis of rheumatoid arthritis.More recently, inhibition of TNF production has been shown to have broadapplication in the treatment of inflammation, inflammatory boweldisease, Alzheimer's disease, Crohn's disease, multiple sclerosis andasthma.

TNF has also been implicated in viral infections, such as HIV, influenzavirus, and herpes virus including herpes simplex virus type-1 (HSV-1),herpes simplex virus type-2 (HSV-2), cytomegalovirus (CMV),varicella-zoster virus (VZV), Epstein-Barr virus, human herpes virus-6(HHV-6), human herpes virus-7 (HHV-7), human herpes virus-8 (HHV-8),pseudorabies and rhinotracheitis, among others.

Similarly, IL-1 is produced by activated monocytes and macrophages, andplays a role in many pathophysiological responses including rheumatoidarthritis, fever and reduction of bone resorption.

The inhibition of these cytokines by inhibition of the p38 kinase is ofbenefit in controlling, reducing and alleviating many of these diseasestates.

SUMMARY OF THE INVENTION

One aspect of the present invention provides compounds represented bythe formula:

wherein:

-   -   A is N or CH;    -   R¹ is hydrogen, alkyl or aralkyl;    -   R² is alkyl, heteroalkyl, (R″)₂NCO-alkylene-(where each R″ is        independently hydrogen or alkyl), cycloalkyl, heterocyclyl,        aryl, or heteroaryl;    -   X is O, N(R³) or S, wherein R³ is hydrogen, alkyl or aryl;    -   Y is a bond, O, N(R′), C(═O), CH(OR′), CHR′, or S(O)_(n),        wherein n is 0, 1, or 2; and R′ is hydrogen or alkyl; and    -   R is aryl or heteroaryl; and        isomers, pharmaceutically acceptable salts, esters or prodrugs        thereof.

The compounds of formula I and their pharmaceutically acceptable saltsare inhibitors of protein kinases and exhibit effective activity againstp38 in vivo. Therefore, the compounds can be used for the treatment ofdiseases mediated by the pro-inflammatory cytokines such as TNF andIL-1.

Thus, in another aspect, the present invention relates to methods forthe treatment of p38 mediated diseases or conditions in which atherapeutically effective amount of a compound of formula I isadministered to a patient in need of such treatment.

In yet another aspect, the present invention relates to methods forpreparing the compounds described above.

In yet still another aspect, the present invention relates to methodsfor preparing medicaments useful for the treatment of the p38 mediateddiseases and conditions.

In yet another aspect of the invention, there are provided compoundsuseful as intermediates in preparing compounds of formula (I).

Definitions

As used herein, the term “alkyl” means a linear or branched saturatedmonovalent hydrocarbon moiety of one to six carbon atoms, e.g., methyl,ethyl, n-propyl, 2-propyl, tert-butyl, pentyl, and the like.“Alkylene”means a linear or branched saturated divalent hydrocarbon moiety of oneto six carbon atoms, e.g., methylene, ethylene, propylene, and the like.

The term “aryl” refers to a monovalent monocyclic or bicyclic aromatichydrocarbon moiety which is optionally substituted independently withone or more, preferably one, two or three, substituents. Preferably,each substituent is independently selected from the group consisting ofalkyl, haloalkyl, halo, hydroxy, amino, haloalkoxy, cyano, nitro,heteroalkyl, methylenedioxy, ethylenedioxy, —Y-aryl, —Y-heteroaryl,—Y-cycloalkyl, —Y-heterocyclyl, —Y—OR^(p), —Y—NR^(p)R^(q),—Y—C(O)—R^(p), —YS(O)₀₋₂R^(p), —Y—N—S(O)₂R^(p), —Y—S(O)₂NR^(p)R^(q),—Y—N—C(O)NR^(p)R^(q), where Y is absent or a C₁–C₃ alkylene group, andR^(p) and R^(q) are independently selected from hydrogen, alkyl,haloalkyl, hydroxy, alkoxy, aryl, heteroaryl, cycloalkyl, andheterocyclyl, except when said substituent is —YS(O)₁₋₂R^(p) or—Y—N—S(O)₂R^(p), then R^(p) in these instances is not hydrogen. Aparticularly preferred group of aryl substituents are those selectedfrom alkyl, haloalkyl, halo, hydroxy, amino, haloalkoxy and heteroalkyl.Within this group of aryl substituents, halide, alkyl and alkoxy areespecially preferred. More specifically the term aryl includes, but isnot limited to, phenyl, chlorophenyl, methoxyphenyl, 1-naphthyl,2-naphthyl, and the derivatives thereof.

“Aralkyl” means a moiety of the formula —R^(x)R^(y) where R^(x) is analkylene group and R^(y) is an aryl group as defined above. Exemplaryaralkyls include benzyl, phenylethylene, and the like.

The term “cycloalkyl” as used herein refers to a saturated monovalentcyclic hydrocarbon moiety of three to seven ring carbons, e.g.,cyclopentyl, cyclobutyl, cyclohexyl, and the like. Cycloalkyl mayoptionally be substituted with one, two or three substituents.Preferably, each substituent is independently selected from the groupconsisting of alkyl, hydroxy, alkoxy, amino, monosubstituted amino,disubstituted amino, haloalkyl, halo, cyanoalkyl, oxo (i.e., carbonyloxygen), heteroalkyl, heterocyclyl, hydroxyalkyl, and —(X)_(n)—C(O)R′(where, X is O or NR″, n is 0 or 1, R″ is hydrogen, alkyl, haloalkyl,amino, monosubstituted amino, disubstituted amino, hydroxy, alkoxy,alkyl or optionally substituted phenyl, and R′ is H or alkyl), and—S(O)_(n)R′ (wherein n is 0 to 2 provided where n is 1 or 2, R′ is nothydrogen). A particularly preferred group of cycloalkyl substituents arethose selected from alkyl, hydroxy, alkoxy, amino, monosubstitutedamino, disubstituted amino, haloalkyl and halo. Among this group ofcycloalkyl substituents, alkyl, hydroxy, alkoxy, haloalkyl and halo areespecially preferred. More specifically, the term cycloalkyl includescyclopentyl, cyclohexyl, 4-hydroxycyclohexyl, and the like.

The term “halo,” “halide” or “halogen,” when referring to a substituentmeans fluoro, chloro, bromo, or iodo.

The term “haloalkyl” means alkyl substituted with one or more same ordifferent halo atoms, e.g., —CH₂Cl, —CF₃, —CH₂CF₃, —CH₂CCl₃, and thelike, and further includes those alkyl groups such as perfluoroalkyl inwhich all alkyl hydrogen atoms are replaced by fluorine atoms.

The term “heteroalkyl” as used herein means an alkyl moiety definedabove, wherein one, two or three hydrogen atoms have been replaced witha substituent independently selected from the group consisting of—OR^(a), —NR^(b)R^(c), and S(O)_(n)R^(d) (where n is an integer from 0to 2), with the understanding that the point of attachment of theheteroalkyl moiety is through a carbon atom, wherein R^(a) is hydrogen,acyl, alkyl, cycloalkyl, or cycloalkylalkyl; R^(b) and R^(c) areindependently of each other hydrogen, acyl, alkyl, cycloalkyl, orcycloalkylalkyl; or R^(b) and R^(c) together with the nitrogen atom towhich they are attached form heterocyclyl or heteroaryl; and when n is0, R^(d) is hydrogen, akyl, cycloalkyl, or cycloalkylalkyl, and when nis 1 or 2, R^(d) is alkyl, cylcoalkyl, cycloalkylalkyl, amino,acylamino, monoalkylamino, or dialkylamino. Representative examplesinclude, but are not limited to, 2-hydroxyethyl, 3-hydroxypropyl,2-hydroxymethylethyl, 2,3-dihydroxypropyl, 1-hydroxymethylethyl,3-hydroxybutyl, 2,3-dihydroxybutyl, 2-hydroxy-1-methylpropyl,2-aminoethyl, 3-aminopropyl, 2-methylsulfonylethyl, aminosulfonylmethyl,aminosulfonylethyl, methylaminosulfonylmethyl, methylaminosulfonylethyl,methylaminosulfonylpropyl, and the like. When R^(a) in the moiety—OR^(a)is hydrogen, “heteroalkyl” is also referred to as “hydroxyalkyl” andincludes, but is not limited to, 2-hydroxyethyl, 3-hydroxypropyl,2-hydroxymethylethyl, 2,3-dihydroxypropyl, 1-hydroxymethylethyl,3-hydroxybutyl, 2,3-dihydroxybutyl, and 2-hydroxy-1-methylpropyl.“Monosubstituted amino” means a moiety —NHR^(e) where R^(e) is alkyl,heteroalkyl, haloalkyl, cycloalkyl, cycloalkylalkyl, hydroxyalkyl, aryl,aralkyl, aralkenyl, heteroaryl, heteroaralkyl, heteroaralkenyl,heterocyclyl, or heterocyclylalkyl, e.g., methylamino, ethylamino,phenylamine, benzylamine, and the like. Similarly, the term“disubstituted amino” refers to a moiety —NR^(g)R^(h) wherein R^(g) andR^(h) are, independently of each other, alkyl, heteroalkyl, haloalkyl,cycloalkyl, cycloalkylalkyl, hydroxyalkyl, aryl, aralkyl, aralkenyl,heteroaryl, heteroaralkyl, heteroaralkenyl, heterocyclyl, orheterocyclylalkyl, or R^(g) and R^(h) together with the nitrogen atom towhich they are attached form a heterocyclyl ring. Representativeexamples include, but are not limited to, dimethylamino,methylethylamino, di(1-methyl-ethyl)amino, piperazinyl, piperdinyl andthe like.

“Heterocyclyl” means a saturated or partially-unsaturated non-aromaticcyclic moiety in which one or two ring atoms are heteroatoms selectedfrom N, O, or S(O)_(n) (where n is an integer from 0 to 2), theremaining ring atoms being C, where one or two C atoms may optionallycontain a carbonyl oxygen group, e.g., one or two atoms in the ring maybe a moiety of the formula —C(═O)—. The heterocyclyl ring may beoptionally substituted independently with one, two, or threesubstituents selected from alkyl, hydroxy, hydroxyalkyl, alkoxy,heteroalkyl, and haloalkyl. More specifically the term heterocyclylincludes, but is not limited to, tetrahydropyranyl, piperidinyl,piperazinyl, morpholinyl, and the like.

“Heteroaryl” means a monovalent monocyclic or bicyclic aromatic moietyof 5 to 12 ring atoms containing one, two, or three ring heteroatomseach independently selected from N, O, or S, the remaining ring atomsbeing C. The heteroaryl ring can optionally be substituted with one ormore substituents, preferably one or two substituents, each substituentbeing independently selected from alkyl, haloalkyl, heteroalkyl,heterocyclyl, halo, nitro, cyano, carboxy, acyl, -(alkylene)_(n)—COOR(where n is 0 or 1 and R is hydrogen, alkyl, optionally substitutedphenylalkyl, or optionally substituted heteroaralkyl), or-(alkylene)_(n)—CONR^(a)R^(b) (where n is 0 or 1, and R^(a) and R^(b)are, independently of each other, hydrogen, alkyl, cycloalkyl,cycloalkylalkyl, hydroxyalkyl, aryl, or R^(a) and R^(b) together withthe nitrogen atom to which they are attached form a heterocyclyl orheteroaryl ring). More specifically the term heteroaryl includes, but isnot limited to, pyridyl, furanyl, thiophenyl, thiazolyl, isothiazolyl,triazolyl, imidazolyl, isoxazolyl, pyrrolyl, pyrazolyl, pyrimidinyl,benzofuranyl, isobenzofuranyl, benzothiazolyl, benzoisothiazolyl,benzotriazolyl, indolyl, isoindolyl, benzoxazolyl, quinolyl,isoquinolyl, benzimidazolyl, benzisoxazolyl, benzothiophenyl,dibenzofuranyl, and benzodiazepin-2-one-5-yl, and the derivativesthereof.

The term “acyl” refers to the group —C(O)R^(r) where R^(r) is alkyl,haloalkyl, heteroalkyl, aryl, heteroaryl, aralkyl or heteroaralkyl.

“Alkoxy”, “aryloxy”, “aralkyloxy”, or “heteroaralkyloxy” means a moiety—OR where R is an alkyl, aryl, aralkyl, or heteroaralkyl, respectively,as defined above e.g., methoxy, phenoxy, pyridin-2-ylmethyloxy,benzyloxy, and the like.

“Leaving group” has the meaning conventionally associated with it insynthetic organic chemistry, i.e., an atom or a group capable of beingdisplaced by a nucleophile and includes halo (such as chloro, bromo, andiodo), alkanesulfonyloxy, arenesulfonyloxy, alkylcarbonyloxy (e.g.,acetoxy), arylcarbonyloxy, mesyloxy, tosyloxy,trifluoromethanesulfonyloxy, aryloxy (e.g., 2,4-dinitrophenoxy),methoxy, N,O-dimethylhydroxylamino, and the like.

“Pharmaceutically acceptable excipient” means an excipient that isuseful in preparing a pharmaceutical composition that is generally safe,non-toxic and neither biologically nor otherwise undesirable, andincludes excipients that are acceptable for veterinary use as well ashuman pharmaceutical use. A “pharmaceutically acceptable excipient” asused in the specification and claims includes both one and more than onesuch excipient.

“Pharmaceutically acceptable salt” of a compound means a salt that ispharmaceutically acceptable and that possesses the desiredpharmacological activity of the parent compound. Such salts include: (1)acid addition salts, formed with inorganic acids such as hydrochloricacid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, andthe like; or formed with organic acids such as acetic acid, propionicacid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvicacid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid,fumaric acid, tartaric acid, citric acid, benzoic acid,3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid,2-hydroxyethanesulfonic acid, benzenesulfonic acid,4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid,4-toluenesulfonic acid, camphorsulfonic acid,4-methylbicyclo[2.2.2]-oct-2-ene-1carboxylic acid, glucoheptonic acid,4,4′-methylenebis-(3-hydroxy-2-ene-1-carboxylic acid), 3-phenylpropionicacid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuricacid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylicacid, stearic acid, muconic acid, and the like; or (2) salts formed whenan acidic proton present in the parent compound either is replaced by ametal ion, e.g., an alkali metal ion, an alkaline earth ion, or analuminum ion; or coordinates with an organic base such as ethanolamine,diethanolamine, triethanolamine, tromethamine, N-methylglucamine, andthe like.

The terms “pro-drug” and “prodrug” are used interchangeably herein andrefer to any compound which releases an active parent drug according toFormula I in vivo when such prodrug is administered to a mammaliansubject. Prodrugs of a compound of Formula I are prepared by modifyingone or more functional group(s) present in the compound of Formula I insuch a way that the modification(s) may be cleaved in vivo to releasethe parent compound. Prodrugs include compounds of Formula I wherein ahydroxy, amino, or sulfhydryl group in a compound of Formula I is bondedto any group that may be cleaved in vivo to regenerate the freehydroxyl, amino, or sulfhydryl group, respectively. Examples of prodrugsinclude, but are not limited to, esters (e.g., acetate, formate, andbenzoate derivatives), carbamates (e.g., N,N-dimethylaminocarbonyl) ofhydroxy functional groups in compounds of Formula I, and the like.

“Protecting group” refers to a grouping of atoms that when attached to areactive group in a molecule masks, reduces or prevents that reactivity.Examples of protecting groups can be found in T. W. Green and P. G.Wuts, Protective Groups in Organic Chemistry, (Wiley, 2^(nd) ed. 1991)and Harrison and Harrison et al., Compendium of Synthetic OrganicMethods, Vols. 1–8 (John Wiley and Sons, 1971–1996). Representativeamino protecting groups include, formyl, acetyl, trifluoroacetyl,benzyl, benzyloxycarbonyl (CBZ), tert-butoxycarbonyl (Boc), trimethylsilyl (TMS), 2-trimethylsilyl-ethanesulfonyl (SES), trityl andsubstituted trityl groups, allyloxycarbonyl,9-fluorenylmethyloxycarbonyl (FMOC), nitro-veratryloxycarbonyl (NVOC),and the like. Representative hydroxy protecting groups include thosewhere the hydroxy group is either acylated or alkylated such as benzyl,and trityl ethers as well as alkyl ethers, tetrahydropyranyl ethers,trialkylsilyl ethers and allyl ethers.

“Treating” or “treatment” of a disease includes: (1) preventing thedisease, i.e., causing the clinical symptoms of the disease not todevelop in a mammal that may be exposed to or predisposed to the diseasebut does not yet experience or display symptoms of the disease; (2)inhibiting the disease, i.e., arresting or reducing the development ofthe disease or its clinical symptoms; or (3) relieving the disease,i.e., causing regression of the disease or its clinical symptoms.

“A therapeutically effective amount” means the amount of a compoundthat, when administered to a mammal for treating a disease, issufficient to effect such treatment for the disease. The“therapeutically effective amount” will vary depending on the compound,the disease and its severity and the age, weight, etc., of the mammal tobe treated.

DETAILED DESCRIPTION OF THE INVENTION

In one embodiment, the present invention provides compounds representedby the formula:

wherein A, R¹, R², X, Y and R are as defined above.

Some of the representative compounds of formula I are shown in Table 1below.

TABLE 1 Compounds of formula I, wherein the values of R¹, R², R³, R′, A,X, Y and R are set forth below: I

MS Cpd. # R¹ R² R′ R³ A X Y R MP° C. M + H  1 H 4-hydroxy-cyclohexyl — —N S CO phenyl 102.6– 354 103.8  2 H N-methylsulfonylpiperidin- — — N SCO phenyl 216.3– 417 4-yl 217.6  3 H tetrahydropyran-4-yl — — N S COphenyl 219.5– 340 220.5  4 H tetrahydropyran-4-yl — — N S CO4-fluorophenyl 236– 358 238.7  5 H tetrahydropyran-4-yl — — N S CO2-chlorophenyl — —  6 H N-methylsulfonylpiperidin- — — N S CO2-fluorophenyl 213.3– 435 4-yl 216.1  7 H N-methylsulfonylpiperidin- — —N S CO 3-fluorophenyl 225.4– 435 4-yl 225.7  8 H tetrahydropyran-4-yl —— N S CO 3-fluorophenyl 186.7– 358 188.8  9 H tetrahydropyran-4-yl — — NS CO 2-fluorphenyl 195.2– 358 195.5 10 H (1,1-dimethyl-2- — — N S CO2-fluorphenyl 105- 346 hydroxy)ethyl 108.5 11 H tetrahydropyran-4-yl — —N S CO 4-chlorophenyl 261.4– 374 262.9 12 H N-methylsulfonylpiperidin- —— N S CO 4-fluorophenyl 250.3– 435 4-yl 251.1 13 HN-methylsulfonylpiperidin- — — N S CO 4-chlorophenyl 260.8– — 4-yl 261.814 H N-methylsulfonylpiperidin- — — N S CO 2-chlorophenyl 188.1– 4514-yl 188.5 15 H 1,1-dioxo- — — N S CO 2-fluorophenyl 217.9– 406tetrahydrothiopyran-4-yl 221.8 (See cpd. #36) 16 HN-methylsulfonylpiperidin- — — N S CO 3-fluorophenyl 191– 406 4-yl 193.417 H (1,1-dimethyl-2- — — N S CO 3-fluorophenyl 133.3– 346.1hydroxy)ethyl 134.6 18 H (1-methyl-2-methoxy)ethyl — — N S CO3-fluorophenyl 159.4– 346 167.2 19 H (1-methyl-2-hydroxy)ethyl — — N SCO 3-fluorophenyl 148.6– 332 150.5 20 H (1-methyl-2,2-dimethyl-2- — — NS CO 2-chlorophenyl 106– 376 hydroxy)ethyl 113 21 H 1-(2,2-dimethyl-2- —— N S CO 2-fluorophenyl 104.4– 360 hydroxyethyl)ethyl 117.3 22 HN-methylsulfonylpiperidin- H — N S CH₂ phenyl 215– 403 4-yl 217 23 HN-methylsulfonylpiperidin- H — N S CHO phenyl 152.2– 419 4-yl H 162.1 24H tetrahydropyran-4-yl H — N S CHO 2-fluorophenyl — 360.2 H 25 Htetrahydropyran-4-yl H — N S CH₂ 2-fluorophenyl 217– 344 219 26 Htetrahydropyran-4-yl — — N S CO 2-methyl-phenyl 183– 354 188 27 Htetrahydropyran-4-yl — — N S CO 2-methoxy- 194.4– 370 phenyl 198.4 28 Htetrahydropyran-4-yl — — N S CO 3-methoxy- 206– 370 phenyl 208.6 29 Htetrahydropyran-4-yl H — N O CH₂ phenyl 310.3 30 H cyclopentyl H — N OCH₂ phenyl — 294.2 31 H 4-hydroxycyclohexyl H — N O CH₂ phenyl — 324.232 H tetrahydropyran-4-yl — methyl N N CO 2-methoxy- — 367.2 phenyl 33 Hcyclopentyl H methyl N N CH₂ phenyl — 307.3 34 H 4-hydroxycyclohexyl Hmethyl N N CH₂ phenyl — 337.2 35 H tetrahydropyran-4-yl H methyl N N CH₂phenyl — 323.2 36 H

— — N S CO 4-fluorophenyl >300 406 37 H tetrahydropyran-4-yl — — N O —phenyl 224– 296 226 38 H isopropyl — — N O CH₂ phenyl 268.2 39 H phenyl— phenyl N N — 4-methoxyphenyl 40 H 1-(2-hydroxyethyl)-3- — — N S CO2-methoxyphenyl 388 hydroxypropyl 41 H tetrahydropyran-4-yl — — N S S2,4- 380 difluorophenyl 42 H tetrahydropyran-4-yl — — N S O 2,4- 364difluorophenyl 43 H tetrahydropyran-4-yl — — N S S phenyl 344

In one preferred group of compounds of Formula I, R is aryl. Preferredaryl groups are optionally substituted phenyl. Particularly preferredoptionally substituted phenyls are phenyl (i.e., non-substitutedphenyl), mono- and di-halo substituted phenyls, alkylsubstitutedphenyls, and alkoxysubstituted phenyl. Especially preferred optionallysubstituted phenyls are phenyl, 4-fluorophenyl, 2-chlorophenyl,2-fluorophenyl, 3-fluorophenyl, 4-chlorophenyl, 2-methoxyphenyl,2-methylphenyl, 3-methoxyphenyl, 4-methoxyphenyl, and2,4-difluorophenyl.

In another preferred group of compounds of Formula I, Y is C(═O), CH₂,CH(OH), a bond (i.e., absent), S or O.

Yet in another preferred group of compounds of Formula I, A is N.

Still in another preferred group of compounds of Formula I, R¹ ishydrogen.

Yet in another preferred group of compounds of Formula I, R² isheterocyclyl, cycloalkyl, heteroalkyl, alkyl, or aryl. Within this groupof R², 4-hydroxycyclohexyl, N-methylsulfonylpiperidin-4-yl,tetrahydropyran-4-yl, (1,1-dimethyl-2-hydroxy)ethyl,1,1-dioxo-tetrahydrothiopyran-4-yl, 2-methoxy-1-methylethyl,2-hydroxy-1-methylethyl, 1,2-dimethyl-2-hydroxypropyl, cyclopentyl,isopropyl, phenyl, and 1-(2-hydroxyethyl)-3-hydroxypropyl areparticularly preferred.

Still further, combinations of the preferred groups described hereinform other preferred embodiments. In this manner, a variety of preferredcompounds are embodied within the present invention. Representativegroups of particularly preferred compounds are described below.

One preferred group of compounds of formula I are those wherein Y is CH₂or C(═O); and R is a phenyl optionally substituted with a hydrogen,halogen, methyl, trifluoromethyl, alkoxy, trifluoromethoxy, cyano, nitroor amino.

More preferred compounds in this embodiment are compounds of formula I,wherein A is N, Y is CH₂ or C(═O), and X is S. Still more preferredcompounds are compounds of formula I wherein A is N, Y is C(═O), R¹ ishydrogen and R² is a cycloalkyl, heterocycloalkyl or an aryl.

Particularly preferred compounds of formula I are those wherein A is N,X is S, Y is C(═O) and R is a phenyl optionally substituted withhalogen, alkyl, trifluoromethyl, alkoxy or hydroxyalkyl, R¹ is hydrogenand R² is a cycloalkyl, heterocycloalkyl or an aryl.

Most preferred compounds are those of formula I wherein A is N, X is S,Y is C(═O), R is phenyl substituted with halogen, alkyl,trifluoromethyl, alkoxy or hydroxyalkyl, R¹ is hydrogen and R² is aheterocyclyl group.

In another preferred embodiment of compounds of Formula I, Y is O or S.Within this group of compounds of Formula I, preferably A is N. Morepreferred compounds within this group are those where X is S. Inparticular, compounds where R² is heterocyclyl are still more preferred.Still further preferred compounds within this group are those where R isaryl, in particular where R is optionally substituted phenyl. Moreparticularly, those compounds where R¹ is hydrogen are especiallypreferred.

Still in another preferred embodiment of compounds of Formula I, Y is abond, i.e., R is directly attached to the core heteroaryl group on thecarbon atom adjacent to the X moiety. Particularly preferred compoundswithin this embodiment are those where X is O or NR³. Still morepreferred are those where R² is heterocyclyl or aryl. One specific groupof compounds within this group are those where R³ is phenyl.

Yet in another preferred embodiment of compounds of Formula I, R isaryl, preferably optionally substituted phenyl. Particularly preferredare those where A is N. More preferred compounds within this group ofcompounds of Formula I are those where R¹ is hydrogen. Still morepreferred compounds are those where R² is alkyl, hydroxyalkyl,cycloalkyl, heterocyclyl or aryl, with those where R³ is methyl orphenyl being especially preferred.

In yet another preferred embodiment of compounds of Formula I, A is N.Preferred compounds within this group are those where R is aryl, withcompounds where R² is alkyl, hydroxyalkyl, cycloalkyl, heterocyclyl oraryl being particularly preferred. Especially preferred are those whereR¹ is hydrogen.

The compounds of the present invention can exist in unsolvated forms aswell as solvated forms, including hydrated forms and all such forms areintended to be encompassed within the scope of the invention.Furthermore, as stated above, the present invention also includes allpharmaceutically acceptable salts of the compounds along with prodrugforms of the compounds and all stereoisomers whether in a pure chiralform, a racemic mixture, or enantiomeric mixture form.

The compounds of formula I are capable of further formingpharmaceutically acceptable acid addition salts. As stated above, all ofthese forms are also contemplated within the scope of the claimedinvention.

Pharmaceutically acceptable acid addition salts of the compounds offormula I include salts derived from inorganic acids such ashydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydriodic,phosphorus, and the like, as well as the salts derived from organicacids, such as aliphatic mono- and dicarboxylic acids,phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioicacids, aromatic acids, aliphatic and aromatic sulfonic acids, etc. Suchsalts include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite,nitrate, phosphate, monohydrogenphosphate, dihydrogenphosphate,metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate,propionate, caprylate, isobutyrate, oxalate, malonate, succinate,suberate, sebacate, fumarate, maleate, mandelate, benzoate,chlorobenzoate, methylbenzoate, dinitrobenzoate, pthalate,benzenesulfonate, toluenesulfonate, phenylacetate, citrate, lactate,maleate, tartarate, methanesulfonate, and the like. Also contemplatedare salts of amino acids such as arginate and the like and gluconate,galacturonate (see, for example, Berge et al., “Pharmaceutical Salts,”J. of Pharmaceutical Science, 1977, 66, 1–19).

The acid addition salts of the basic compounds can be prepared bycontacting the free base form with a sufficient amount of the desiredacid to produce the salt in the conventional manner. The free base formcan be regenerated by contacting the salt form with a base and isolatingthe free base in the conventional manner. The free base forms may differfrom their respective salt forms somewhat in certain physical propertiessuch as solubility in polar solvents, but otherwise the salts areequivalent to their respective free base for the purposes of the presentinvention.

Pharmaceutically acceptable base addition salts can be formed with metalions or amines, such as alkali and alkaline earth metal ions or organicamines. Examples of metal ions which are used as cations include sodium,potassium, magnesium, calcium, and the like. Examples of suitable aminesare N,N′-dibenzylethylenediamine, chlororocaine, choline,diethanolamine, ethylenediamine, N-methylglucamine, and procaine (see,for example, Berge et a., supra).

The base addition salts of acidic compounds can be prepared bycontacting the free acid form with a sufficient amount of the desiredbase to produce the salt in the conventional manner. The free acid formcan be regenerated by contacting the salt form with an acid andisolating the free acid in the conventional manner. The free acid formsmay differ from their respective salt forms somewhat in certain physicalproperties such as solubility in polar solvents, but otherwise the saltsare equivalent to their respective free acid for the purposes of thepresent invention.

All patents, patent applications and publications cited in thisapplication are hereby incorporated by reference in their entirety forall purposes to the same extent as if each individual patent, patentapplication or publication were so individually denoted.

Processes for Preparing the Compounds

The compounds of the present invention can be prepared by a variety ofmethods, using procedures well-known to those of skill in the art. Thefollowing schemes illustrate the processes of making the compounds ofthe invention.

Abbreviations

The abbreviations used herein have the following meaning:

-   MCPBA: m-chloroperbenzoic acid.-   NMP: N-methylpyrrolidine.-   THF: tetrahydrofuran.-   TLC: thin layer chromatography.-   EtOAc: ethyl acetate.    Scheme 1

Scheme 1 describes the method of preparing a compound of formula I(a)and its analogs I(b) and I(c).

Treatment of a compound of formula 1 with potassium fluoride provides acompound of formula 2, which is then converted to a carboxaldehyde 3according to the literature procedure (Ple, N.; Turck, A.; Heynderickx,A.; Queguiner, G. J.; Heterocyclic Chem. 1994, 31, 1311). Carboxaldehyde3 can be coupled with the substituted phenacylthiols without furtherpurification to give a compound of formula formula 4. The reaction istypically carried out with triethylamine as the base at about 0° C. toabout room temperature.

Oxidation of compound 4 with an oxidizing agent, such as3-chloroperbenzoic acid (i.e., MCPBA) or Oxone®, provides a sulfone 5which can be converted into a variety of target compounds. Typically theoxidation of 5 is carried out in a solvent which is inert under theconditions of the oxidation. For example, when MCPBA is used as theoxidizing agent, the solvent is preferably a halogenated aliphatichydrocarbon, especially dichloromethane.

When Oxone® is used an the oxidizing agent, the solvent is typically amixture of water and tetrahydrofuran. The reaction temperature dependson the solvent used. For an organic solvent, the reaction temperature isgenerally at about −20° C. to about 50° C., preferably about 0° C. toabout room temperature. When water is used as the solvent, the reactiontemperature is generally from about 0° C. to about 50° C., preferablyabout 0° C. to about room temperature.

Reaction of compound 5 with an amine of formula R¹R²NH, where R¹ and R²are as defined above, affords a compound of formula I(a). The reactioncan be carried out in the presence or absence of a solvent.Conveniently, the reaction is carried out at temperatures of from about0° C. to about 200° C., more preferably about room temperature to about150° C.

Compound I(a) can be reduced to the alcohol of formula I(b) by carryingout the reduction with a reducing agent, such as sodium borohydride inethanol at room temperature. Compound of formula I(b) can in turn beconverted to a compound of formula I(c). This reduction is usuallycarried out in dichloromethane with triethylsilane and trifluoroaceticacid.

Alternatively (not shown here), reaction of compound 5 with ammoniaprovides a compound of formula I(a)′ (i.e., compound of formula I, whereR¹ and R² are hydrogen). Further alkylation of I(a)′ then providescompounds of formula I, where R¹ and/or R² are not hydrogen. Thereaction can be carried out in the presence or absence of solvent.Conveniently, the reaction is carried out at temperatures of from about0° C. to about 200° C., more preferably about room temperature to about150° C. Alternatively (not shown here), in some cases rather than usingthe sulfone 5, the sulfide 4 can be reacted directly with an amine(R¹R²NH) to provide the compounds of formula I(a).

Scheme 2

Scheme 2 illustrates a method of preparing the pyrrole analog ofcompound I(a). The condensation of the amino aldehyde 6 with asubstituted phenacyl bromide gives compound 7. The reaction is typicallycarried out in NMP at 80° C. Compound 7 is then oxidized to sulfone 8 bythe method described above under Scheme 1. Sulfone 8 is then reactedwith an appropriate amine as described above to afford compound offormula II.

Scheme 3

A furan analog of compound of formula I(a) can be prepared according toScheme 3. A compound 8 (prepared according to methods described inSakamoto, T.; Kondo, Y.; Watanabe, R.; Yamanaka, H.; Chem. Pharm. Bull.1986, 34, 2719) can be converted to a compound 9 by palladium catalyzedcoupling with alkynes and cyclization (see: Sakamoto, T.; Kondo, Y.;Watanabe, R.; Yamanaka, H.; Chem. Pharm. Bull. 1986, 34, 2719). Compound9 is then oxidized to a compound 10 by methods described above. Compound10 is then reacted with a desirable amine of formula HNR¹R² to afford acompound of formula III.

Scheme 3A

Alternatively, compound 8 can be converted into a silyl furanopyrimidineof formula 11 which can be coupled with aryl aldehyde to give an alcoholof formula 12 by the procedure described in the literature. (Aquila, B.M;. Tetrahedron Lett. 1997, 38, 2795). Compound 12 is then convertedinto a sulfone 13 which can then be converted to a compound of formulaIII (a) by reaction with an amine of formula HNR¹R². Compound III(a) isthen converted into other derivatives thereof as described above inScheme 1 and also by a number of routes available to those skilled inthe art.

Scheme 4

A compound of formula IV can be prepared according to Scheme 4. Acompound of formula 14 (prepared according to methods described inSakamoto, T.; Kondo, Y.; Watanabe, R.; Yamanaka, H.; Chem. Pharm. Bull.1986, 34, 2719) is converted to a compound of formula 15 via a palladiumcatalyzed coupling reaction with an alkyne. Compound 15 is then treatedwith methylamine to afford compound 16. The cyclization of compound 16to compound 17 can be accomplished by treatment with a mixture of CuIand PdCl₂(PPh₃)₂. Compound 17 is then converted to sulfone 18 andfinally to a compound of formula IV by methods described in the previousschemes.

Scheme 5

Compounds of formulae Va and Vb can be prepared according to Scheme 5. Acompound of formula 5-1 is reacted with α-cyanoacetamide of formula 5-2to afford a thiophene compound of formula 5-3. The thiophene compound5-3 is then reacted with a potassium salt of dithiocarbonic acidO-propyl ester of formula 5-4 to produce a thienopyrimidin-4-one offormula 5-5. Methylation of the thienopyrimidin-4-one of formula 5-5with methyl iodide then gave a corresponding thioether of formula 5-6.Oxidation and simultaneous chlorination of the thioether of formula 5-6is then achieved using POCl₃ to afford thienopyrimidine of formula 5-7.Oxidation of the thioether of formula 5-7 to a sulfonyl compound offormula 5-8 is achieved using an oxidizing agent, e.g., Oxone. Thechloro-substituent on the pyrimidine ring system is then reduced byhydrogenation using palladium on carbon catalyst to afford2-methanesulfonyl-thieno[2,3-d]pyrimidine of formula 5-9. Themethanesulfonyl group of formula 5-9 can be replaced with a desiredamine compound to afford a desired amino-substituent. For example, asshown in Scheme 5, the methanesulfonyl group is displaced with4-aminotetrahydropyran of formula 5-10 to yield2-(tetrahydropyran-4-yl)amino-substituted thienopyrimidine of formula5-11. This thienopyrimidine of formula 5-11 is then halogenated, e.g.,using mercury (II) oxide and iodine, to afford iodo-substitutedthienopyrimidine of formula 5-12. The iodo-substituted thienopyrimidineof formula 5-12 can be reacted with a variety of compounds, such asphenols (e.g., compound of formula 5-13, and its correspondingthiophenol compound, not shown) and or metalated by treatment with anorganometallic reagent (e.g. alkyl lithiums) and subsequent reactionwith disulfides (e.g., compound of formula 5-14), to yield correspondingcoupled products, e.g., compounds of formulas Va and Vb, respectively.

One of skill in the art will understand that certain modifications tothe above schemes are contemplated and within the scope of the presentinvention. For example, certain steps will involve the use of protectinggroups for functional groups that are not compatible with particularreaction conditions.

Pharmaceutical Compositions Containing the Compounds

The compounds of formula I and the pharmaceutically acceptable salts ofcompounds of formula I can be used as medicaments, e.g. in the form ofpharmaceutical preparations. The pharmaceutical preparations can beadministered enterally, e.g. orally in the form of tablets, coatedtablets, dragees, hard and soft gelatine capsules, solutions, emulsionsor suspensions, nasally, e.g. in the form of nasal sprays, or rectally,e.g. in the form of suppositories. However, they may also beadministered parenterally, e.g. in the form of injection solutions.

The compounds of formula I and their aforementioned pharmaceuticallyacceptable salts can be processed with pharmaceutically inert, organicor inorganic carriers for the production of pharmaceutical preparations.Lactose, corn starch or derivatives thereof, talc, stearic acid or itssalts and the like can be used, for example, as such carriers fortablets, coated tablets, dragees and hard gelatine capsules. Suitablecarriers for soft gelatine capsules are, for example, vegetable oils,waxes, fats, semi-solid and liquid polyols and the like; depending onthe nature of the active ingredient no carriers are, however, usuallyrequired in the case of soft gelatine capsules. Suitable carriers forthe production of solutions and syrups are, for example, water, polyols,sucrose, invert sugar, glucose and the like. Suitable carriers forsuppositories are, for example, natural or hardened oils, waxes, fats,semi-liquid or liquid polyols and the like.

The pharmaceutical preparations can also contain preservatives,solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners,colorants, flavorants, salts for varying the osmotic pressure, buffers,masking agents or antioxidants. They can also contain therapeuticallyvaluable substances other than the compounds of formula I and theiraforementioned pharmaceutically acceptable salts.

Medicaments which contain a compound of formula I or a pharmaceuticallyacceptable salt of a basic compound of formula I with an acid inassociation with a compatible pharmaceutical carrier material are alsoan object of the present invention, as is a process for the productionof such medicaments which comprises bringing one or more of thesecompounds or salts and, if desired, one or more other therapeuticallyvaluable substances into a galenical administration form together with acompatible pharmaceutical carrier.

As mentioned earlier, the compounds of formula I and theiraforementioned pharmaceutically acceptable salts can be used inaccordance with the invention as therapeutically active substances,especially as antiinflammatory agents or for the prevention of graftrejection following transplant surgery. The dosage can vary within widelimits and will, of course, be fitted to the individual requirements ineach particular case. In general, in the case of administration toadults a convenient daily dosage should be about 0.1 mg/kg to about 100mg/kg, preferably about 0.5 mg/kg to about 5 mg/kg. The daily dosage maybe administered as a single dose or in divided doses and, in addition,the upper dosage limit referred to earlier may be exceeded when this isfound to be indicated.

Finally, the use of compounds of formula I and their aforementionedpharmaceutically acceptable salts for the production of medicaments,especially in the treatment or prophylaxis of inflammatory,immunological, oncological, bronchopulmonary, dermatological andcardiovascular disorders, in the treatment of asthma, central nervoussystem disorders or diabetic complications or for the prevention ofgraft rejection following transplant surgery, is also an object of theinvention.

Methods of Using the Compounds and Compositions

Compounds of Formula I are useful for, but not limited to, the treatmentof any disorder or disease state in a human, or other mammal, which isexacerbated or caused by excessive or unregulated TNF or p38 kinaseproduction by such mammal. Compounds of Formual I inhibit p38 kinase inin vitro assays and inhibit TNF-α release in cell based assaysinhibiting as described in Examples 12 and 13. Accordingly, the presentinvention provides a method of treating a cytokine-mediated diseasewhich comprises administering an effective cytokine-interfering amountof a compound of Formula I, or a pharmaceutically acceptable salt or atautomer thereof.

Compounds of Formula I are useful for, but not limited to, the treatmentof inflammation in a subject, and for use as antipyretics for thetreatment of fever. Compounds of the invention are also useful intreating arthritis, including but not limited to, rheumatoid arthritis,spondyloarthropathies, gouty arthritis, osteoarthritis, systemic lupuserythematosus and juvenile arthritis, and other arthritic conditions. Inaddition, compounds of the present invention are useful in treatingpulmonary disorders or lung inflammation, including adult respiratorydistress syndrome, pulmonary sarcoidosis, asthma, silicosis, and chronicpulmonary inflammatory disease. Furthermore, compounds of the presentinvention are also useful in treating viral and bacterial infections,including sepsis, septic shock, gram negative sepsis, malaria,meningitis, cachexia secondary to infection or malignancy, cachexiasecondary to acquired immune deficiency syndrome (AIDS), AIDS, ARC (AIDSrelated complex), pneumonia, and herpes virus. Moreover, compounds ofthe present invention are also useful in the treatment of boneresorption diseases, such as osteoporosis, endotoxic shock, toxic shocksyndrome, reperfusion injury, autoimmune disease including graft vs.host reaction and allograft rejections, cardiovascular diseasesincluding atherosclerosis, thrombosis, congestive heart failure, andcardiac reperfusion injury, renal reperfusion injury, liver disease andnephritis, and myalgias due to infection.

The compounds of the present invention are also useful for the treatmentof influenza, multiple sclerosis, cancer, diabetes, systemic lupuserthrematosis (SLE), skin-related conditions such as psoriasis, eczema,bums, dermatitis, keloid formation, and scar tissue formation. Compoundsof the present invention are also useful in treating gastrointestinalconditions such as inflammatory bowel disease, Crohn's disease,gastritis, irritable bowel syndrome and ulcerative colitis. Thecompounds of the present invention can also be used in treatingophthalmic diseases, such as retinitis, retinopathies, uveitis, ocularphotophobia, and of acute injury to the eye tissue. Compounds of theinvention also would be useful for treatment of angiogenesis, includingneoplasia; metastasis; ophthalmological conditions such as corneal graftrejection, ocular neovascularization, retinal neovascularizationincluding neovascularization following injury or infection, diabeticretinopathy, retrolental fibroplasia and neovascular glaucoma;ulcerative diseases such as gastric ulcer; pathological, butnon-malignant, conditions such as hemangiomas, including infantilehemangiomas, angiofibroma of the nasopharynx and avascular necrosis ofbone; diabetic nephropathy and cardiomyopathy; and disorders of thefemale reproductive system such as endometriosis. In addition, compoundsof the present invention are also useful for preventing the productionof cyclooxygenase-2.

Besides being useful for human treatment, compounds of the presentinvention are also useful for veterinary treatment of companion animals,exotic animals and farm animals, including mammals, rodents, and thelike. More preferred animals include horses, dogs, and cats.

Furthermore, compounds of the present invention can also be used inco-therapies, partially or completely, in place of other conventionalantiinflammatories, such as together with steroids, cyclooxygenase-2inhibitors, NSAIDs, DMARDS, immunosuppressive agents, 5-lipoxygenaseinhibitors, LTB₄ antagonists and LTA₄ hydrolase inhibitors.

As used herein, the term “TNF mediated disorder” refers to any and alldisorders and disease states in which TNF plays a role, either bycontrol of TNF itself, or by TNF causing another monokine to bereleased, such as but not limited to IL-1, IL-6 or IL-8. A disease statein which, for instance, IL-1 is a major component, and whose productionor action, is exacerbated or secreted in response to TNF, wouldtherefore be considered a disorder mediated by TNF.

As used herein, the term “p38 mediated disorder” refers to any and alldisorders and disease states in which p38 plays a role, either bycontrol of p38 itself, or by p38 causing another factor to be released,such as but not limited to IL-1, IL-6 or IL-8. A disease state in which,for instance, IL-1 is a major component, and whose production or action,is exacerbated or secreted in response to p38, would therefore beconsidered a disorder mediated by p38.

As TNF-β has close structural homology with TNF-β (also known ascachectin), and since each induces similar biologic responses and bindsto the same cellular receptor, the synthesis of both TNF-α and TNF-β areinhibited by the compounds of the present invention and thus are hereinreferred to collectively as “TNF” unless specifically delineatedotherwise.

EXAMPLES

The following examples and the methods of preparation of the compoundsare given to enable those skilled in the art to more clearly understandand practice the present invention. These examples are merelyillustrative and are not intended to be limiting.

Example 12-(tetrahydropyran-4-ylamino)-6-(2-chlorobenzoyl)thieno[2,3-d]pyrimidine

This example illustrates the preparation of a compound of formula I bythe method described under scheme 1.

Step 1

4-fluoro-2-methylthiopyrimidine

To a solution of 35.7 g (0.22 mol) of 4-chloro-2-methylthiopyrimidine(Aldrich Chemical Co., Milwaukee, Wis., USA) in 135 mL of tetraglyme wasadded 18-crown-6 (1.33 g) and potassium fluoride (Anhydrous, AldrichChemical Co., Milwaukee, Wis., USA, 80 g). The mixture was heated at150° C.with stirring for 16 hours. The mixture was then cooled anddistilled under reduced pressure to give 20 g (62%) of4-fluoro-2-methylthiopyrimidine as a liquid.

Step 2

4-fluoro-2-methylthiopyrimidine-5-carboxaldehyde

To THF (100 mL) at −78° C. was added 2.5 M n-butyl lithium (20.4 mL).The mixture was warmed to 0° C. and diisopropyl amine (8.2 mL) was addedslowly. The mixture was stirred at 0° C. for 30 minutes and then cooledto −78° C. A solution of 4-fluoro-2-methylthiopyrimidine (3.76 g, 22.2mmol) in 5 mL of THF was slowly added and the mixture was stirred for 2hours. Ethyl formate (4.3 mL, 44.4 mmol) was added and stirred foradditional 2 hours. 4 N HCl (25 mL), ethanol (25 mL), and THF (100 mL)were slowly added and stirred for additional 10 min after the coolingbath was removed. The mixture was diluted with ethyl acetate (250 mL),water (200 mL), and saturated sodium bicarbonate (100 mL). The organiclayer was separated and washed with brine (2×50 mL), dried over MgSO₄,and filtered. The solvent was removed to give a viscous oil (5.66 g).

Step 3

2-(methylthio)-6-(aroyl)thieno[2,3-d]pyrimidine

To a 0° C. solution of the crude4-fluoro-2-methylthiopyrimidine-5-carboxaldehyde obtained in step 2 (2.8g, approximately 11 mmol) and triethyl amine (1.44 mL) in THF (40 mL)was slowly added a solution of 2′-chloro-2-thioacetophenone (1.93 g) inTHF (30 mL), (prepared as described in Step 3A below). The mixture wasthen slowly warmed to room temperature and stirred overnight. Theresulting solution was added to a mixture of ethylacetate (500 mL) andwater (250 mL). The organic layer was separated, washed with brine andsodium bicarbonate solution, dried, and evaporated. The crude productwas purified by column chromatography (silica gel, 5–10% EtOAc/hexane)to give the desired product (1.82 g), MP 137–140.4° C.; MS: 321 (M+H).

Step 3A

2′-chloro-2-thioacetophenone

A mixture of 2′-chloroacetophenone (25 g, 0.162 mol) and copper bromide(72.8 g) in ethyl acetate (90 mL) and chloroform (90 mL) was refluxedfor 2 hours. The mixture was cooled to room temperature, filteredthrough a pad of celite and washed with ethyl acetate. The solvents wereremoved to give crude 2-bromo-2′-chloroacetophenone (35 g).

A mixture of the above bromide (14.67 g) and potassium thioacetate (7.5g) in acetone (250 mL) was stirred at room temperature overnight. Anadditional 3.7 g of possium thioacetate was added and the mixture wasstirred for another day. The reaction mixture was filtered and washedwith acetone. The filtrate was evaporated, and the residue was dilutedwith ethyl acetate (200 mL) and brine (150 mL). The organic layer wasseparated, dried, and evaporated to give crude2-(acetylthiol)-2′-chloroacetophenone (14.4 g).

To a solution of the above product (2.29 g, 10 mmol) in methanol (100mL) was slowly added a solution of sodium thiomethoxide (0.7 g, 10 mmol)in methanol (10 mL). The reaction mixture was stirred for 2 hours andpoured into 200 mL of 0.1 M HCl and extracted with dichloromethane(2×150 mL). The organic phase was washed with brine (150 mL), dried, andevaporated to give the crude 2′-chloro-2-thioacetophenone (1.93 g).

Step 4

2-(methanesulfonyl)-6-(aroyl)thieno[2,3- d]Pyrimidine

To a solution of the sulfide (1.6 g) obtained in Step 3 in THF (80 mL)was added a solution of Oxone (Aldrich, 6.13 g) in water (40 mL) at 0°C. The mixture was then stirred at room temperature for 5 hours. Ethylacetate (250 mL) and water (150 mL) were added. The organic phase wasseparated, washed with water (2×150 mL), dried, and evaporated to givethe sulfone (1.56 g), MP: 164.1–165.4° C. MS: 352.9 (M+H).

Step 5

2-(tetrahydropyran-4-ylamino)-6-(2-chlorobenzoyl)thieno[2,3-d]pyrimidine

A mixture of the sulfone obtained above (352 mg) and4-aminotetrahydropyran (152 mg) in NMP (0.1 mL) was heated at 100° C.for 3 hours. Ethyl acetate (180 mL) and water (50 mL) were added. Theorganic layer was separated, washed with brine, dried, and evaporated.The crude product was purified by preparative TLC (silica gel, 65%EtOAc/hexanes) to give 253 mg of the final product. It was converted tothe hydrochloride salt by treatment with 1.5 equivalents of 1N HCl inether. MS: 374 (M+H).

Example 22-(tetrahydropyran-4-ylamino)-6-(2-fluorobenzoyl)thieno[2,3-d]pyrimidine

The above compound was prepared in a similar manner as described inexample 1.

Example 2A2-(tetrahydropyran-4-ylamino)-6-[(2-fluorophenyl)hydroxylmethyl]thieno[2,3-d]pyrimidine

To a solution of2-(tetrahydropyran-4-ylamino)-6-(2-fluorobenzoyl)thieno[2,3-d]pyrimidine(300 mg) in ethanol (30 mL) was added sodium borohydride (0.4 g) at roomtemperature and stirred overnight. Ethyl acetate (50 mL) was added tothe reaction mixture. The organic layer was separated, washed withbrine, dried, and evaporated. Purification by preparative TLC (silicagel, 50% EtOAc/hexanes) gave 140 mg of the alcohol. MS: 360.2 (M+H).

Example 2B2-(tetrahydropyran-4-ylamino)-6-[(2-fluorophenyl)methyl]thieno[2,3-d]pyrimidine

The alcohol (140 mg) obtained in Example 2A was stirred withtriethylsilane (1.0 mL) and trifluoroacetic acid (1.5 mL) indichloromethane (5 mL) for 4 hours. The solvents were removed. Theresidue was diluted with toluene (5 mL) and then concentrated. Thisdilution-concentration process was repeated three times. Purificationwith preparative TLC (50% EtOAc/Hexanes) gave the final product. It wasconverted to the hydrochloride salt with 1N HCl in ether to yield 65 mgof the salt. MP: 217–219° C. MS: 344 (M+H).

Example 3

2-(tetrahydropyran-4-ylamino)-6-(2-methoxybenzoyl)-7-methyl-pyrrolo[2,3-d]pyrimidine

This example illustrates the method of making a compound of formula Iaccording to the method described in Scheme 2.

Step 1

Ethyl 4-methylamino-2-methylthiopyrimidine-5-carboxylate

To a 0° C. solution of 20 g (86 mmol) of ethyl4-chloro-2-methylthiopyrimidine-5-carboxylate (Aldrich Chemical Co.,Milwaukee, Wis., USA) in 250 mL of dichloromethane was slowly added 35mL (281 mmol) of a 33% solution of methylamine in ethanol. Afterstirring for 30 minutes, 150 mL of water was added and the phases wereseparated. The organic phase was dried over magnesium sulfate andfiltered. The filtrate was evaporated under reduced pressure to give 19g (97%) of ethyl 4-methylamino-2-methylthiopyrimidine-5-carboxylate as awhite solid.

Step 2

4-methylamino-2-methylthiopyrimidine-5-methanol

To a suspension of lithium aluminum hydride (9 g, 237 mmol) in 300 mL ofdry tetrahydrofuran was added dropwise a solution of 34 g (143 mmol) ofethyl 4-methylamino-2-methylthiopyrimidine-5-carboxylate in 300 mL ofdry tetrahydrofuran and left to stand for 15 minutes. The mixture wascooled in an ice bath and 18 mL of water was added dropwise followed by36 mL of 2 M sodium hydroxide solution and 48 mL of additional water.The resulting suspension was stirred for 17 hours at room temperatureand then filtered. The filter residue was washed twice with 100 mL ofethyl acetate, and the combined filtrate and washings were evaporatedunder reduced pressure. The residue was suspended in 200 mL ofdichloromethane/hexane (2:1) and the solid was filtered and dried togive 23.5 g (86%) of 4-methylamino-2-methylthiopyrimidine-5-methanol asa yellow solid.

Step 3

4-methylamino-2-methylthiopyrimidine-5-carboxaldehyde

To a solution of 4-methylamino-2-methylthiopyrimidine-5-methanol (20 g,108 mmol) in 1 L of dichloromethane was added 87 g (1 mol) of manganesedioxide. The resulting suspension was stirred for 24 hours and thenfiltered through a filter aid. The filter residue was washed with 100 mLof dichloromethane and the combined filtrate and washings wereevaporated under reduced pressure to give 15.8 g (80%) of4-methylamino-2-methylthiopyrimidine-5-carboxaldehyde as a white solid.

Step 4

2-(methylthio)-6-(2-methoxybenzoyl)-7-methyl-pyrrolo[2,3-d]pyrimidine

A mixture of the aldehyde (0.792 g), 2′-methoxy bromoacetophenone (1.17g, Aldrich) and potassium carbonate (1.6 g) in NMP (5mL) was stirred at70–80° C. overnight. Additional 2′-methoxy bromoacetophenone (0.88 g)was added and the mixture was stirred at 100° C. overnight. Additional2′-methoxy bromoacetophenone (0.65 g) and potassium carbonate (1.12 g)were added and the mixture was again stirred at 100° C. overnight. Thereaction mixture was cooled and diluted with ethyl acetate (150 mL) andwashed with brine (3×50 mL), dried, and concentrated. Columnchromatography (silica gel, 10–30% EtOAc/hexanes) followed bypreparative TLC (silica gel, 30% EtOAc/hexanes) gave 100 mg of theproduct. MS: 334 (M+H).

Step 5

2-(tetrahydropyran-4-ylamino)-6-(2-methoxybenzoyl)-7-methyl-pyrrolo[2,3-d]pyrimidine

The sulfide (0.1 g) obtained in the Step 4 above was stirred with oxone(0.5 g) in THF/H₂O (2 mL/2 mL) for 4 hours. Aqueous workup with ethylacetate and brine gave the crude sulfone.

The sulfone obtained above was heated with 4-aminotetrahydropyran (0.18g) in NMP (0.2 mL) at 140° C. overnight. An aqueous work up gave thecrude product. Purification by preparative TLC gave the desired product(2 mg). MS: 367 (M+H).

Example 4 2-(cyclopentylamino)-6-benzyl-furano[2,3-d]pyrimidine

This example illustrates the preparation of a compound of formula Iaccording to that described under Scheme 3.

Step 1

2-(methylthio)-6-benzyl-furano[2,3-d]pyrimidine

A mixture of 2-methylthio-4-hydroxy-5-iodopyrimidine (2.65 g),3-phenyl-1-propyne (1.49 mL), copper (I) iodide (90 mg) andbis(triphenylphosphine) Palladium (II) dichloride (Fluka, 160 mg) in 20mL of triethylamine and NMP (7 mL) was stirred at 40° C. for 6 hours.The reaction mixture was diluted with ethyl acetate (100 mL), washedwith brine (3×50 mL), concentrated and purified by column chromatography(5% EtOAc/hexanes) to give 0.57 g of a solid product. MP: 69–72.2° C.MS: 257.2 (M+H).

Step 2

2-(cyclopentylamino)-6-benzyl-furano[2,3-d]pyrimidine

A solution of the sulfide (0.57 g) obtained in Step 1 above in THF (15mL) was stirred with oxone (2.2 g) in water (15 mL) at 0° C. for 3 hoursand at room temperature for 1 hour. An aqueous work up gave the crudesulfone (containing sulfoxide) (0.63 g).

A solution of the sulfone (60 mg) thus obtained in 1 mL ofaminocyclopentane was stirred at 100° C. for 2 hours. The excessaminocyclopentane was removed. The crude product was purified bypreparative TLC (40% EtOAc/hexanes) to give 47 mg of a solid product.MS: 294.2 (M+H).

Example 52-(cyclopentylamino)-6-benzyl-7-methyl-pyrrolo[2,3-d]pyrimidine

This example illustrates the preparation of2-(cyclopentylamino)-6-benzyl-7-methyl-pyrrolo[2,3-d]pyrimidineaccording to that described in Scheme 4.

Step 1

2-methylthio-5-(3-phenylpropyn-1-yl)-6-methylaminopyrimidine

A mixture of 2-methanethio-4-chloro-5-iodopyrimidine (6.5 g),3-phenyl-1-propyne (3.4 mL), copper (I) iodide (130 mg),bis(triphenylphosphine)palladium(II) dichloride (322 mg), andtriethylamine (25 mL) was heated in NMP at 40° C. for 2 hours. Themixture was cooled to 0° C. and 25 mL of 40% methylamine (Aldrich) inacetonitrile (15 mL) was added. The resulting mixture was stirred atroom temperature for 2 hours and then concentrated. The residue wasdiluted with ethyl acetate (200 mL) and washed with brine (3×100 mL).The organic layer was separated, dried and evaporated to give an oil.Column chromatography purification (5–12% EtOAc/hexanes) gave 3.8 g of asolid product. MS: 270 (M+H).

Step 2

2-(methylthio)-6-benzyl-7-methyl-pyrrolo[2,3-d]pyrimidine

A mixture of the alkyne obtained above (3.47 g), triethylamine (15 mL),copper (I) iodide (200 mg), bis(triphenylphosphine)palladium dichloride(200 mg) in NMP (10 mL) was refluxed for 6 hours. Aqueous work up gave 4g of dark residue. Column chromatography purification (10–13%EtOAc/hexanes) gave 1.0 g of the product. MS: 270.2 (M+H).

Step 3

2-(methanesulfinyl)-6-benzyl-7-methyl-pyrrolo[2,3-d]pyrimidine

The sulfide (0.3 g) in THF (10 mL) was stirred with a solution of oxone(0.9 g) in water (10 mL) at 0–5° C.for 3 hours. Aqueous work up gave thesulfoxide (containing sulfone).

Step 4

2-(cyclopentylamino)-6-benzyl-7-methyl-pyrrolo[2,3-d]pyrimidine

A mixture of the sulfoxide (50 mg) from Step 3 and cyclopentylamine (1mL) was stirred at 100° C. overnight. Excess cyclopentylamine wasremoved and the residue was purified by preparative TLC (25%EtOAc/hexanes) to give the desired product (26 mg). MS: 307.3 (M+H).

Example 6 (Tetrahydropyran-4-yl)-thieno[23-d]pyrimidin-2-yl-amine

Step 12-Amino-thiophene-3-carboxlic acid amide

2,5-Dihydroxy-1,4-dithiane (76 g) and cyanoacetamide (84 g) were addedto a mixture of methanol (180 mL), water (10 mL) and triethylamine (10g). The resulting mixture was heated at 35–40° C. for about 30 minuteswhile stirring, and then heated to 50–60° C. for an additional 30minutes with stirring. The reaction mixture was then cooled to roomtemperature and poured into a mixture of ice (100 g)/water (300 mL). Afine precipitate formed upon addition, which was filtered and driedovernight to give 100.6 g of the title compound as a pale gray powder((M+H)⁺=143, M.P.=159.0–159.6° C.).

Step 2

2-Thioxo-2,3-dihydro-1H-thieno[2,3-d]pyrimidin-4-one

2-Amino-thiophene-3-carboxlic acid amide (28.4 g, 0.2 mol) and potassiumethylxanthate (96 g, 3 eq) were mixed together and added to DMF (1000mL). The resulting mixture was heated to 150° C. for about six hours.The solvent (DMF) was removed on the rotovap under high vacuum at 90° C.The residue was diluted with 600 mL of aqueous citric acid (5%) andcooled to 0° C. and stirred for about 30 minutes. The tan powder wasfiltered and dried overnight to give 25.6 g of the title compound((M+H)⁺=185, M.P.>300° C.).

Step 3

2-Methylsulfanyl-3H-thieno[2,3-d]pyrimidin-4-one

To a solution of 2-thioxo-2,3-dihydro-1H-thieno[2,3-d]pyrimidin-4-one(25.4 g, 0.138 mol) in 1N aqueous NaOH (600 mL) at room temperature wasadded methyl iodide (10.3 mL, 1.2 eq). The resulting mixture was stirredvigorously for about 2.5 hours. The reaction mixture was cooled to 0° C.and acetic acid was added (about 80 mL) until about pH=4.5 was reached.A fine precipitate was filtered and dried overnight to afford the titlecompound as a fine tan powder (24.8 g) (M⁺=198, M.P.=231.6–235.0° C.).

Step 4

4—Chloro-2-methylsulfanyl-thieno[2,3-d]pyrimidine

2-Methylsulfanyl-3H-thieno[2,3-d]pyrimidin-4-one (12 g, 60.5 mmol) wascombined with POCl₃ (56 mL), and the resulting mixture was heated toreflux for about 1 hour. The reaction mixture was concentrated underreduced pressure at 50° C. The residue was diluted with ethyl acetate(700 mL) at 0° C. Saturated sodium bicarbonate solution (600 mL) wasadded slowly. The resulting mixture was stirred vigorously at 0° C. forone hour and the layers were separated. Saturated sodium bicarbonatesolution (600 mL) was added to the organic layer at 0° C., and themixture was stirred vigorously for 20 minutes. The layers wereseparated. Brine (600 mL) was added to the organic layer and stirredvigorously for 5 minutes, and the layers were again separated. Theorganic layer was dried over magnesium sulfate, filtered andconcentrated to afford the title compound (10.7 g) as a dark tan powder(M⁺=216, M.P.=105.0–107.4° C.).

Step 5

4—Chloro-2-methanesulfonyl-thieno[2,3-d]pyrimidine

To a solution of 4-chloro-2-methylsulfanyl-thieno[2,3-d]pyrimidine (10g, 46.15 mmol) in tetrahydrofuran (350 mL) at 0° C. was added a solutionof OXONE (59.6 g, 2.1 eq) in water (300 mL) dropwise with stirring. Theresulting mixture was gradually warmed from 0° C. to room temperatureovernight. The reaction mixture was diluted with ethyl acetate (1000 mL)and water (300 mL), and the layers were separated. The aqueous layer wasextracted with ethyl acetate (1×300 mL). Organic layers were combined,washed with brine (2×300 mL), dried over magnesium sulfate, filtered andconcentrated to give 10.7 g of the title compound as a tan powder(M+H)⁺=249.

Step 6

2-Methanesulfonyl-thieno[2,3-d]pyrimidine

Nitrogen gas was bubbled through a solution of4-chloro-2-methanesulfonyl-thieno[2,3-d]pyrimidine (2.8 g) in ethanol(400 mL) and tetrahydrofuran (75 mL) for 5 minutes. To this solution wasadded 10% palladium on activated charcoal (2.8 g). The resulting mixturewas placed on a Parr shaker under 30 psi of hydrogen gas. After 16hours, additional catalyst (1 g) was added, and the mixture was againplaced on the Parr shaker under 30 psi hydrogen gas for additional 6hours. The reaction mixture was filtered through a 3 cm bed of celite.The filter cake was washed with dichloromethane. Concentration of thefiltrate gave the title compound (2.2 g).

Step 7

(Tetrahydropyran-4-yl)-thieno[2,3-d]pyrimidin-2-yl-amine

A mixture of 2-methanesulfonyl-thieno[2,3-d]pyrimidine (1.58 g, 7.37mmol), 4-aminotetrahydropyran (2.24 g, 3 eq) and 1-methyl-2-pyrolidinone(2 mL) was heated to 100° C. with stirring for about 7 hours andcontinued heating at 80° C. overnight. The reaction mixture was cooledto room temperature and diluted with ethyl acetate (180 mL)/water (60mL). The layers were separated, and the organic layer was washedsuccessively with water (4×60 mL) and brine (1×60 mL). The organic layerwas then dried over magnesium sulfate, filtered and concentrated to give1.7 g of the crude product. Purification by column chromatography onsilica gel eluting with 25% ethyl acetate in hexanes afforded the titlecompound as a white powder (1.264 g) ((M+H)⁺=236, M.P.=127.0–130.0° C.).

Example 76-Iodo-thieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine

Step 16-Iodo-thieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine

To a solution of(tetrahydro-pyran-4-yl)-thieno[2,3-d]pyrimidin-2-yl-amine (786 mg, 3.34mmol) in benzene (40 mL) was added mercury(II) oxide (854 mg, 1.18 eq)followed by iodine (1.00 g, 1.18 eq). The resulting mixture was stirredvigorously at room temperature overnight. Additional mercury(II) oxide(427 mg, 0.6 eq) and iodine (500 mg, 0.6 eq) were added, and the mixturewas again stirred vigorously at room temperature for one additional day.The reaction mixture was then filtered through a 3 cm bed of celite andwashed with ethyl acetate (180 mL). The filtrate was washed successivelywith saturated aqueous solution of Na₂S₂O₃ (4×50 mL), water (2×50 mL)and brine (1×50 mL). The organic layer was dried over magnesium sulfate,filtered and concentrated to give the title compound as a reddish powder(823 mg) (M+H)⁺=362.

Example 8[6-(2,4-Difluorophenoxy)thieno[2,3-d]pyrimidin-2-yl]-(tetrahydropyran-4-yl)-amine

Step 1[6-(2.4-Difluorophenoxy)thieno[2,3-d]pyrimidin-2-yl]-(tetrahydropyran-4-yl)-amine

A mixture of 2,4-difluorophenol (0.5 mL, 38 eq), sodium hydride (205 mg,37 eq) and 1-methyl-2-pyrolidinone (0.6 mL) in a 2.5 mL microwavereactor vessel was stirred for about 5 minutes. To this mixture wasadded 6-iodo-thieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine(50 mg, 0.14 mmol), and the resulting mixture was placed on a microwavereactor for 75 minutes at 150° C. The reaction mixture was then cooledto room temperature, diluted with ethyl acetate (85 mL) and washedsuccessively with water (3×30 mL) and brine (1×30 mL). The ethyl acetatelayer was concentrated and then purified by Preparative Thin LayerChromatography eluting on two (20×20 cm, 1000 μM) silica gel plates with40% ethyl acetate in hexanes. The title compound was isolated as anoff-white powder (8 mg) (M+H)⁺=364.

Example 9[6-(2,4-Difluorophenylsulfanyl)thieno[2,3-d]pyrimidin-2-yl]-(tetrahydropyran-4-yl)-amine

Step 1[6-(2,4-Difluorophenylsulfanyl)thieno[2,3-d]pyrimidin-2-yl]-(tetrahydropyran-4-yl)-amine

2,4-Difluorobenzenethiol (0.35 mL, 10.8 eq) was added to6-iodo-thieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine (100mg, 0.277 mmol), and the resulting mixture was stirred overnight at roomtemperature. The reaction mixture was then heated 100° C. with stirringfor 5 hours, after which 1-methyl-2-pyrolidinone (0.2 mL) was addedfollowed by potassium carbonate (415 mg, 10.8 eq). The resulting mixturewas heated with stirring to 150° C. for an additional 5 hours. Thereaction mixture was then cooled to room temperature and diluted withethyl acetate (35 mL)/water (20 mL). The layers were separated. Theorganic layer was washed with successively with water (3×20 mL) andbrine (1×20 mL), dried over magnesium sulfate, filtered and concentratedto yield a crude product (452 mg), which was purified by PreparativeThin Layer Chromatography eluting on four (20×40 cm, 1000 μM) silica gelplates with 55% ethyl acetate in hexanes to afford the title compound(16 mg) as an off-white powder (M+H)⁺=380.

Example 10(6-Phenylsulfanylthieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine

Step 1(6-Phenylsulfanylthieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine

To a solution of6-iodothieno[2,3-d]pyrimidin-2-yl)-(tetrahydropyran-4-yl)-amine (100 mg,0.277 mmol) in tetrahydrofuran (3 mL) at −78° C. was added 2.5Mn-butyllithium in hexanes (0.23 mL, 2.1 eq) dropwise. The reactionmixture turned from red to yellow. The resulting mixture was stirred at−78° C. for 45 minutes before slowly adding a solution of phenyldisulfide (242 mg, 4 eq) in tetrahydrofuran (3 mL) at −78° C. Thereaction was gradually warmed from −78° C. to room temperature over 4hours and quenched by adding water (5 mL). The resulting mixture wasdiluted with ethyl acetate (35 mL)/water (25 mL). The layers werepartitioned, and the separated organic layer was washed with brine (1×20mL), dried over magnesium sulfate, filtered and concentrated to give thecrude product. Purification by Preparative Thin Layer Chromatographyeluting on two (20×20 cm, 1000 μM) silica gel plates with 50% ethylacetate in hexanes afforded the title compound (16 mg) as an off-whitepowder (M+H)⁺=344.

Example 11

The following are representative pharmaceutical formulations containinga compound of Formula (I).

Tablet Formulation

The following ingredients are mixed intimately and pressed into singlescored tablets.

Quantity per Ingredient tablet, mg compound of this invention 400cornstarch 50 croscarmellose sodium 25 lactose 120 magnesium stearate 5

Capsule Formulation

The following ingredients are mixed intimately and loaded into ahard-shell gelatin capsule.

Quantity per Ingredient capsule, mg compound of this invention 200lactose, spray-dried 148 magnesium stearate 2

Suspension Formulation

The following ingredients are mixed to form a suspension for oraladministration.

Ingredient Amount compound of this invention 1.0 g fumaric acid 0.5 gsodium chloride 2.0 g methyl paraben 0.15 g propyl paraben 0.05 ggranulated sugar 25.5 g Sorbitol (70% solution) 12.85 g Veegum K(Vanderbilt Co.) 1.0 g flavoring 0.035 ml colorings 0.5 mg distilledwater q.s. to 100 ml

Injectable Formulation

The following ingredients are mixed to form an injectable formulation.

Ingredient Amount compound of this invention 0.2 g sodium acetate buffersolution, 0.4 M 2.0 ml HC1 (1N) or NaOH (1N) q.s. to suitable pH water(distilled, sterile) q.s. to 20 ml

All of the above ingredients, except water, are combined and heated to60–70° C. with stirring. A sufficient quantity of water at 60° C. isthen added with vigorous stirring to emulsify the ingredients, and waterthen added q.s. to 100 g.

Suppository Formulation

A suppository of total weight 2.5 g is prepared by mixing the compoundof the invention with Witepsol® H-15 (triglycerides of saturatedvegetable fatty acid; Riches-Nelson, Inc., N.Y.), and has the followingcomposition:

compound of the invention 500 mg Witepsol ® H-15 balance

Example 12 Inhibition Of p-38 (MAP) Kinase-In Vitro Assay

The p-38 MAP kinase inhibitory activity of compounds of this inventionin vitro was determined by measuring the transfer of the y-phosphatefrom γ-³³P-ATP by p-38 kinase to Myelin Basic Protein (MBP), using the aminor modification of the method described in Ahn, N. G.; et al. J. ofBiol. Chem. Vol. 266(7), 4220–4227, (1991)

The phosphorylated form of the recombinant p38 MAP kinase was expressedwith SEK-1 and MEKK in E. Coli (see, Khokhlatchev, A. et al. J. of Biol.Chem. Vol. 272(17), 11057–11062, (1997) and then purified by affinitychromatography using a Nickel column.

The phosphorylated p38 MAP kinase was diluted in kinase buffer (20 mM3-(N-morpholino)propanesulfonic acid, pH 7.2, 25 mM β-glycerolphosphate, 5 mM ethylene glycol-bis(beta-aminoethylether)-N,N,N′,N′-tetraacetic acid, 1 mM sodium vanadate, 1 mMdithiothreitol, 40 mM magnesium chloride). Test compound dissolved inDMSO or only DMSO (control) was added and the samples were incubated for10 min. at 30° C. The kinase reaction was initiated by the addition of asubstrate cocktail containing MBP and γ-³³P-ATP. After incubating for anadditional 20 min. at 30° C., the reaction was terminated by adding0.75% phosphoric acid. The phosphorylated MBP was then separated fromthe residual γ-³³P-ATP using a phosphocellulose membrane (Millipore,Bedfrod, Mass.) and quantitated using a scintillation counter (Packard,Meriden, Conn.).

The p-38 inhibitory activities (expressed as IC₅₀, the concentrationcausing 50% inhibition of the p-38 enzyme being analyzed) of thecompounds listed in Table 1 in the specification are between 0.01 μM and10 μM. Selected data are shown below.

Compound Structure p38 IC₅₀ (nM)  1

104  3

221 23

128 30

744 33

135 41

226

Example 13

This example illustrates an in vitro assay to evaluate the inhibition ofLPS-induced TNF-α production in THP1 cells.

The ability of the compounds of this invention to inhibit the TNF-αrelease was determined using a minor modification of the methodsdescribed in Blifeld, et al. Transplantation, 51:498–503 (1991).

(a) Induction of TNF Biosynthesis:

THP-1 cells were suspended in culture medium [RPMI (Gibco-BRL,Gailthersburg, Md.) containing 15% fetal bovine serum, 0.02 mM2-mercaptoethanol], at a concentration of 2.5×10⁶ cells/mL and thenplated in 96 well plate (0.2 mL aliquots in each well). Test compoundswere dissolved in DMSO and then diluted with the culture medium suchthat the final DMSO concentration was 5%. Twenty five μL aliquots oftest solution or only medium with DMSO (control) were added to eachwell. The cells were incubated for 30 min., at 37° C. LPS (Sigma, St.Louis, Mo.) was added to the wells at a final concentration of 0.5μg/ml, and cells were incubated for an additional 2 h. At the end of theincubation period, culture supernatants were collected and the amount ofTNF-α present was determined using an ELISA assay as described below.

(b) ELISA Assay:

The amount of human TNF-α present was determined by a specific trappingELISA assay using two anti-TNF-α antibodies (2TNF-H12 and 2TNF-H34)described in Reimund, J. M., et al. GUT. Vol. 39(5), 684–689 (1996).

Polystyrene 96-well plates were coated with 50 μl per well of antibody2TNF-H12 in PBS (10 μg/mL) and incubated in a humidified chamber at 4°C. overnight. The plates were washed with PBS and then blocked with 5%nonfat-dry milk in PBS for 1 hour at room temperature and washed with0.1% BSA (bovine serum albumin) in PBS.

TNF standards were prepared from a stock solution of human recombinantTNF-α (R&D Systems, Minneapolis, Minn.). The concentration of thestandards in the assay began at 10 ng/mL followed by 6 half log serialdilutions.

Twenty five μL aliquots of the above culture supernatants or TNFstandards or only medium (control) were mixed with 25 μL aliquots ofbiotinylated monoclonal antibody 2TNF-H34 (2 μg/mL in PBS containing0.1% BSA) and then added to each well. The samples were incubated for 2hr at room temperature with gentle shaking and then washed 3 times with0.1% BSA in PBS. 50 μl of peroxidase-streptavidin (Zymed, S. SanFrancisco, Calif.) solution containing 0.416 μg/mL ofperoxidase-streptavidin and 0.1% BSA in PBS was added to each well. Thesamples were incubated for an additional 1 hr at room temperature andthen washed 4 times with 0.1% BSA in PBS. Fifty μL of O-phenylenediaminesolution (1 μg/mL O-phenylene-diamine and 0.03% hydrogen peroxide in0.2M citrate buffer pH 4.5) was added to each well and the samples wereincubated in the dark for 30 min., at room temperature. Optical densityof the sample and the reference were read at 450 nm and 650 nm,respectively. TNF-α levels were determined from a graph relating theoptical density at 450 nm to the concentration used.

The IC₅₀ value was defined as the concentration of the test compoundcorresponding to half-maximal reduction in 450 nm absorbance.

The foregoing discussion of the invention has been presented forpurposes of illustration and description. The foregoing is not intendedto limit the invention to the form or forms disclosed herein. Althoughthe description of the invention has included description of one or moreembodiments and certain variations and modifications, other variationsand modifications are within the scope of the invention, e.g., as may bewithin the skill and knowledge of those in the art, after understandingthe present disclosure. It is intended to obtain rights which includealternative embodiments to the extent permitted, including alternate,interchangeable and/or equivalent structures, functions, ranges or stepsto those claimed, whether or not such alternate, interchangeable and/orequivalent structures, functions, ranges or steps are disclosed herein,and without intending to publicly dedicate any patentable subjectmatter. All publications, patents, and patent applications cited hereinare hereby incorporated by reference in their entirety for all purposes.

1. A compound represented by Formula V:

wherein: A is N; X is O, NR³ or S, wherein R³ is hydrogen, alkyl oraryl; and Y is a bond, O, NR′, C(═O), CH(OR′), CH(R′), or S(O)_(n),wherein n is 0, 1, or 2; and R′ is hydrogen or alkyl; R is aryl orheteroaryl; and L is R^(a)—S(O)_(n1), where R^(a) is alkyl or aryl andn1 is 0, 1 or 2 wherein aryl is unsubstituted or substituted with 1, 2,or 3 substituents selected from the group alkyl, haloalkyl, halo,hydroxy, amino, haloalkoxy, cyano, nitro, heteroalkyl, methylenedioxy,ethylenedioxy, —Y-aryl, —Y-heteroaryl, —Y-cycloalkyl, —Y-heterocyclyl,—Y—OR^(p), —Y—NR^(p)R^(q), —Y—C(O)—R^(p), —YS(O)₀₋₂R^(p),—Y—N—S(O)₀₋₂R^(p), —Y—S(O)₂NR^(p)R^(q), —Y—N—C(O)NR^(p)R^(q), where Y isa bond or C₁–C₃ alkylene, R^(p) and R^(q) are each independentlyselected from hydrogen, alkyl, haloalkyl, hydroxy, alkoxy, aryl,heteroaryl, cycloalkyl, and heterocyclyl, with the proviso that when thesubstituent is —YS(O)₁₋₂R^(p) or —Y—N—S(O)₂R^(p) said R^(p) is nothydrogen; heteroalkyl is unsubstituted or substituted with 1 or 2substituents selected from the group alkyl, haloalkyl, heteroalkyl,heterocyclyl, halo nitro, cyano, carboxy, acyl, —(alkylene)_(n)—COOR,and —(alkylene)_(n)—CONR^(a)R^(b), where n is 0 or 1, R is hydrogen,alkyl, optionally substituted phenylalkyl, or optionally substitutedheteroaralkyl, R^(a) and R^(b) are each independently hydrogen, alkyl,cycloalkyl, cycloalkylalkyl, hydroxyalkyl, aryl, or R^(a) and R^(b)together with the nitrogen atom to which they are attached formheterocyclyl or heteroaryl.
 2. The compound of claim 1, wherein n1 is 2.3. The compound of claim 2, wherein X is S.
 4. A compound of Formula VI

wherein: A is N; R¹ is hydrogen, alkyl or arylalkyl; R² is alkyl,heteroalkyl, (R″)₂NCO-alkylene- (where each R″ is independently hydrogenor alkyl), cycloalkyl, heterocyclyl, aryl, or heteroaryl; X is S; and Zis hydrogen or halo wherein aryl is unsubstituted or substituted with 1,2, or 3 substituents selected from the group alkyl, haloalkyl, halo,hydroxy, amino, haloalkoxy, cyano, nitro, heteroalkyl, methylenedioxy,ethylenedioxy, —Y-aryl, —Y-heteroaryl, —Y-cycloalkyl,—Y-heterocyclyl,—Y—OR^(p), —Y—NR^(p)R^(q), —Y—C(O)—R^(p), —YS(O)₀₋₂R^(p),—Y—N—S(O)₀₋₂R^(p), —Y—S(O)₂NR^(p)R^(q), —Y—N—C(O)NR^(p)R^(q), where Y isa bond or C₁–C₃ alkylene, R^(p) and R^(q) are each independentlyselected from hydrogen, alkyl, haloalkyl, hydroxy, alkoxy, aryl,heteroaryl, cycloalkyl, and heterocyclyl, with the proviso that when thesubstituent is —YS(O)₁₋₂R^(p) or —Y—N—S(O)₂R^(p) said R^(p) is nothydrogen; heteroaryl is unsubstituted or substituted with 1 or 2substituents selected from the group alkyl, haloalkyl, heteroalkyl,heterocyclyl, halo nitro, cyano, carboxy, acyl, —(alkylene)_(n)—COOR,and —(alkylene)_(n) —CONR^(a)R^(b), where n is 0 or 1, R is hydrogen,alkyl, optionally substituted phenylalkyl, or optionally substitutedheteroaralkyl, R^(a) and R^(b) are each independently hydrogen, alkyl,cycloalkyl, cycloalkylalkyl, hydroxyalkyl, aryl, or R^(a) and R^(b)together with the nitrogen atom to which they are attached formheterocyclyl or heteroaryl.
 5. The compound of claim 4, wherein: R¹ ishydrogen; and R² is alkyl, hydroxyalkyl, cycloalkyl, heterocyclyl oraryl.
 6. A process for producing a compound of the formula:

wherein A is N; R is aryl or heteroaryl; R¹ is hydrogen, alkyl orarylalkyl; R² is alkyl, heteroalkyl, (R″)₂NCO-alkylene- (where each R″is independently hydrogen or alkyl), cycloalkyl, heterocyclyl, aryl, orheteroaryl; X is O, NR³ or S, wherein R³ is hydrogen, alkyl or aryl; andY¹ is C(═O), CH(OR′), or CH(R′), wherein R′ is hydrogen or alkyl, saidprocess comprising: a) contacting a thioether of the formula:

wherein A, R, X and Y¹ are as defined above; and R^(a) is alkyl or aryl,with an oxidizing agent to produce an alkylsulfone compound of theformula:

and b) contacting the alkylsulfone compound with an amine compound ofthe formula NHR¹R² to produce the compound of Formula IA wherein aryland arylalkyl are each independently unsubstituted or substituted with1, 2, or 3 substituents selected from the group alkyl, haloalkyl, halo,hydroxy, amino, haloalkoxy, cyano, nitro, heteroalkyl, methylenedioxy,ethylenedioxy, —Y-aryl, —Y-heteroaryl, —Y-cycloalkyl,—Y-heterocyclyl,—Y—OR^(p), —Y—NR^(p)R^(q), —Y—C(O)—R^(p), —YS(O)₀₋₂R^(p),—Y—N—S(O)₀₋₂R^(p), —Y—S(O)₂NR^(p)R^(q), —Y—N—C(O)NR^(p)R^(q), where Y isa bond or C₁–C₃ alkylene, R^(p) and R^(q) are each independentlyselected from hydrogen, alkyl, haloalkyl, hydroxy, alkoxy, aryl,heteroaryl, cycloalkyl, and heterocyclyl, with the proviso that when thesubstituent is —YS(O)₁₋₂R^(p) or —Y—N—S(O)₂R^(p) said R^(p) is nothydrogen; heteroalkyl is unsubstituted or substituted with 1, 2, or 3substituents selected from the group —OR^(a), —NR^(h)R^(c), andS(O)_(n)R^(d) where n is an integer from 0 to 2, R^(a), R^(b), and R^(c)are each independently hydrogen, acyl, alkyl, cycloalkyl, orcycloalkylalkyl, or R^(b) and R^(c) together with the N to which theyare bound form heterocyclyl or heteroaryl, and R^(d) is hydrogen, acyl,alkyl, cycloalkcyl, or cycloalkylalkyl when n is 0, and R^(d) is alkyl,cycloalkyl, cycloalkylalkyl, amino, acylamino, monoalkylamino, ordialkylamino when n is 1 or 2; cycloalkyl is unsubstituted orsubstituted with 1, 2, or 3 substituents selected from the group alkyl,hydroxy, alkoxy, amino, monosubstituted amino, disubstituted amino,haloalkyl, halo, cyanoalkyl, oxo, heteroalkyl, heterocyclyl,hydroxyalkyl, —S(O)₀₋₂R′, and —(X)_(n)—C(O)R″, where X is O or NR″, n is0 or 1, R″ is hydrogen, alkyl, haloalkyl, amino, monosubstituted amino,disubstituted amino, hydroxy, alkoxy, alkyl or optionally substitutedphenyl, and R′ is H or alkyl; heterocyclyl is unsubstituted orsubstituted with 1, 2, or 3 substituents selected from the group alkyl,hydroxy, hydroxyalkyl, alkoxy, heteroalkyl, and haloalkyl, and 0–2 ringcarbon atoms are substituted with oxo; heteroaryl is unsubstituted orsubstituted with 1 or 2 substituents selected from the group alkyl,haloalkyl, heteroalkyl, heterocyclyl, halo, nitro, cyano, carboxy, acyl,-(alkylene)_(n)-COOR, and -(alkylene)_(n) —CONR^(a)R^(b), where n is 0or 1, R is hydrogen, alkyl, optionally substituted phenylalkyl, oroptionally substituted heteroaralkyl, R^(a) and R^(b) are eachindependently hydrogen, alkyl, cycloalkyl, cycloalkylalkyl,hydroxyalkyl, aryl, or R^(a) and R^(b) together with the nitrogen atomto which they are attached form heterocyclyl or heteroaryl.
 7. A processof producing a compound of Formula IB:

wherein A is N; R is aryl or heteroaryl; R¹ is hydrogen, alkyl orarylalkyl; R² is alkyl, heteroalkyl, (R″)₂NCO-alkylene- (where each R″is independently hydrogen or alkyl), cycloalkyl, heterocyclyl, aryl, orheteroaryl; X is O, NR³ or S, wherein R³ is hydrogen, alkyl or aryl; andY¹ is O, NR′, or S(O)_(n), wherein n is 0, 1, or 2; and R′ is hydrogenor alkyl; said process comprising contacting a compound of the formula:

wherein A, X, R¹ and R² are as defined above; and Z is halide, with anaromatic or heteroaromatic compound of the formula HY¹—R, optionally inthe presence of a base, to produce the compound of Formula IB, where Y¹is O, NR′, or S, and when Y¹ is S, said process further comprising:contacting the compound of Formula IB, where Y¹ is S with an oxidizingagent to produce the compound of Formula IB, where Y¹ is S(O)_(n), wheren is 1 or 2 wherein aryl and arylalkyl are each independentlyunsubstituted or substituted with 1, 2, or 3 substituents selected fromthe group alkyl, haloalkyl, halo, hydroxy, amino, haloalkoxy, cyano,nitro, heteroalkyl, methylenedioxy, ethylenedioxy, —Y-aryl,—Y-heteroaryl, —Y-cycloalkyl,—Y-heterocyclyl, —Y—OR^(p), —Y—NR^(p)R^(q),—Y—C(O)—R^(p), —YS(O)₀₋₂R^(p), —Y—N—S(O)₀₋₂R^(p), —Y—S(O)₂NR^(p)R^(q),—Y—N—C(O)NR^(p)R^(q), where Y is a bond or C₁–C₃ alkylene, R^(p) andR^(q) are each independently selected from hydrogen, alkyl, haloalkyl,hydroxy, alkoxy, aryl, heteroaryl, cycloalkyl, and heterocyclyl, withthe proviso that when the substituent is —YS(O)₁₋₂R^(p) or—Y—N—S(O)₂R^(p) said R^(p) is not hydrogen; heteroalkyl is unsubstitutedor substituted with 1, 2, or 3 substituents selected from the group—OR^(a), —NR^(h)R^(c), and S(O)_(n)R^(d) where n is an integer from 0 to2, R^(a), R^(b), and R^(c) are each independently hydrogen, acyl, alkyl,cycloalkyl, or cycloalkylalkyl, or R^(b) and R^(c) together with the Nto which they are bound form heterocyclyl or heteroaryl, and R^(d) ishydrogen, acyl, alkyl, cycloalkcyl, or cycloalkylalkyl when n is 0, andR^(d) is alkyl, cycloalkyl, cycloalkylalkyl, amino, acylamino,monoalkylamino, or dialkylamino when n is 1 or 2; cycloalkyl isunsubstituted or substituted with 1, 2, or 3 substituents selected fromthe group alkyl, hydroxy, alkoxy, amino, monosubstituted amino,disubstituted amino, haloalkyl, halo, cyanoalkyl, oxo, heteroalkyl,heterocyclyl, hydroxyalkyl, —S(O)₀₋₂R′, and —(X)_(n)—C(O)R″, where X isO or NR″, n is 0 or 1, R″ is hydrogen, alkyl, haloalkyl, amino,monosubstituted amino, disubstituted amino, hydroxy, alkoxy, alkyl oroptionally substituted phenyl, and R′ is H or alkyl; heterocyclyl isunsubstituted or substituted with 1, 2, or 3 substituents selected fromthe group alkyl, hydroxy, hydroxyalkyl, alkoxy, heteroalkyl, andhaloalkyl, and 0–2 ring carbon atoms are substituted with oxo;heteroaryl is unsubstituted or substituted with 1 or 2 substituentsselected from the group alkyl, haloalkyl, heteroalkyl, heterocyclyl,halo, nitro, cyano, carboxy, acyl, -(alkylene)_(n)-COOR, and-(alkylene)_(n) —CONR^(a)R^(b), where n is 0 or 1, R is hydrogen, alkyl,optionally substituted phenylalkyl, or optionally substitutedheteroaralkyl, R^(a) and R^(b) are each independently hydrogen, alkyl,cycloalkyl, cycloalkylalkyl, hydroxyalkyl, aryl, or R^(a) and R^(b)together with the nitrogen atom to which they are attached formheterocyclyl or heteroaryl.
 8. A process of producing a compound ofFormula IC:

wherein n is 0, 1 or 2; A is N; R is aryl or heteroaryl; R¹ is hydrogen,alkyl or arylalkyl; R² is alkyl, heteroalkyl, (R″)₂NCO-alkylene- (whereeach R″ is independently hydrogen or alkyl), cycloalkyl, heterocyclyl,aryl, or heteroaryl; and X is O, NR³ or S, wherein R³ is hydrogen, alkylor aryl; said process comprising: (a) contacting a compound of theformula:

wherein R¹, R², A and X are as defined above; and Z is halide, with anorganometallic reagent; and (b) adding an aryl or heteroaryl disulfideof the formula: R—S—S—R to produce a thioether compound of the formula:

and when n is 1 or 2, said process further comprising: (c) contactingthe thioether compound with an oxidizing agent to produce the compoundof Formula IC where n is 1 or 2 wherein aryl and arylalkyl are eachindependently unsubstituted or substituted with 1, 2, or 3 substituentsselected from the group alkyl, haloalkyl, halo, hydroxy, amino,haloalkoxy, cyano, nitro, heteroalkyl, methylenedioxy, ethylenedioxy,—Y-aryl, —Y-heteroaryl, —Y-cycloalkyl,—Y-heterocyclyl, —Y—OR^(p),—Y—NR^(p)R^(q), —Y—C(O)—R^(p), —YS(O)₀₋₂R^(p), —Y—N—S(O)₀₋₂R^(p),—Y—S(O)₂NR^(p)R^(q), —Y—N—C(O)NR^(p)R^(q), where Y is a bond or C₁–C₃alkylene, R^(p) and R^(q) are each independently selected from hydrogen,alkyl, haloalkyl, hydroxy, alkoxy, aryl, heteroaryl, cycloalkyl, andheterocyclyl, with the proviso that when the substituent is—YS(O)₁₋₂R^(p) or —Y—N—S(O)₂R^(p) said R^(p) is not hydrogen;heteroalkyl is unsubstituted or substituted with 1, 2, or 3 substituentsselected from the group —OR^(a), —NR^(h)R^(c), and S(O)_(n)R^(d) where nis an integer from 0 to 2, R^(a), R^(b), and R^(c) are eachindependently hydrogen, acyl, alkyl, cycloalkyl, or cycloalkylalkyl, orR^(b) and R^(c) together with the N to which they are bound formheterocyclyl or heteroaryl, and R^(d) is hydrogen, acyl, alkyl,cycloalkcyl, or cycloalkylalkyl when n is 0, and R^(d) is alkyl,cycloalkyl, cycloalkylalkyl, amino, acylamino, monoalkylamino, ordialkylamino when n is 1 or 2; cycloalkyl is unsubstituted orsubstituted with 1, 2, or 3 substituents selected from the group alkyl,hydroxy, alkoxy, amino, monosubstituted amino, disubstituted amino,haloalkyl, halo, cyanoalkyl, oxo, heteroalkyl, heterocyclyl,hydroxyalkyl, —S(O)₀₋₂R′, and —(X)_(n)—C(O)R″, where X is O or NR″, n is0 or 1, R″ is hydrogen, alkyl, haloalkyl, amino, monosubstituted amino,disubstituted amino, hydroxy, alkoxy, alkyl or optionally substitutedphenyl, and R′ is H or alkyl; heterocyclyl is unsubstituted orsubstituted with 1, 2, or 3 substituents selected from the group alkyl,hydroxy, hydroxyalkyl, alkoxy, heteroalkyl, and haloalkyl, and 0–2 ringcarbon atoms are substituted with oxo; heteroaryl is unsubstituted orsubstituted with 1 or 2 substituents selected from the group alkyl,haloalkyl, heteroalkyl, heterocyclyl, halo, nitro, cyano, carboxy, acyl,-(alkylene)_(n)-COOR, and -(alkylene)_(n) —CONR^(a)R^(b), where n is 0or 1, R is hydrogen, alkyl, optionally substituted phenylalkyl, oroptionally substituted heteroaralkyl, R^(a) and R^(b) are eachindependently hydrogen, alkyl, cycloalkyl, cycloalkylalkyl,hydroxyalkyl, aryl, or R^(a) and R^(b) together with the nitrogen atomto which they are attached form heterocyclyl or heteroaryl.